Fig. 3From: Improving selectivity of DNA–RNA binding zinc finger using directed evolutionRelative binding of the sequence 5ʹGGGGAAGAA3ʹ by ZfQQR and variants. a Binding affinity measured using surface plasmon resonance on BIAcore 3000 instrument. b The equilibrium dissociation constants (KD) were obtained from global fitting of the results of affinity measurement using the ones site saturation model. c Relative binding in the presence of dsDNA competitor in 100-fold molar excess. 100% is the binding of ZfQQR to substrate with 5ʹGGGGAAGAA3ʹ sequenceBack to article page