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Fig. 1 | BMC Research Notes

Fig. 1

From: Expression of a recombinant bacterial l-asparaginase in human cells

Fig. 1

Bacterial l-asparaginase gene expression in mammalian HEK-293 cells. a pASNase vector, containing two in tandem CMV promoters (pCMV), the coding sequence for the E. coli l-Asparaginase gene (ASNase) with a milk signal peptide sequence (mSP) and a kanamycin resistance gene (kanr). b Transfection of HEK-293 cells in white light (top line) and under UV light (bottom line) with the pASNase vector in order to produce clones with the stably integrated transgene. (1) Cells transfected with the linear pASNase plasmid, (2) Cells transfected without DNA (negative control), (3) Cells transfected with a commercial GFP plasmid (positive control). c Immunodetection of recombinant l-Asparaginase secreted in the medium of HEK-293. (1) Cell culture medium from HEK-293 cells transfected with pASNase plasmid, (2) Cell culture medium from HEK-293 cells transfected with no DNA (negative control), (3) Cellular lysate from HEK-293 cells transfected with pASNase plasmid, (4) Cellular lysate from HEK-293 cells transfected with DNA (negative control), (5) Commercial l-Asparaginase from E. coli (positive control)

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