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Fig. 3 | BMC Research Notes

Fig. 3

From: Crohn’s disease: failure of a proprietary fluorescent in situ hybridization assay to detect M. avium subspecies paratuberculosis in archived frozen intestine from patients with Crohn’s disease.

Fig. 3

“No-probe” negative for a 15-min exposure to 0.2 M HCl. a DAPI; b Texas Red; c Cy-5 (d composite of a–c.) Note the “positive” signal in this “No-probe” control for Fig. 3b–d. Marker bars in µm indicates magnification of × 40. (See Additional file 1 for the With-Probe control for this Figure.) The incubation with HCl is recommended to correct for false positive due to endogenous alkaline phosphatase in the tissue being studied

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BMC Research Notes

ISSN: 1756-0500

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