Fig. 1From: CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibitionDesign and transfection of LAMB1-Dendra2 HDR template into A549 cells. a Diagram of desired insertion of Dendra2. b Linearised sequence map of the LAMB1-Dendra2 HDR template donor, with gRNA protospacer adjacent motifs sites highlighted in pink and linker sequence in yellow. c Gel image from PCR performed on DNA from transfected cells and using primers designed to amplify only when the Dendra2 sequences was inserted in the appropriate genetic location. d Transfected cells fixed with a DAPI counterstain were imaged by confocal analysis. Scale bars represent 20 μmBack to article page