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Fig. 1 | BMC Research Notes

Fig. 1

From: CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition

Fig. 1

Design and transfection of LAMB1-Dendra2 HDR template into A549 cells. a Diagram of desired insertion of Dendra2. b Linearised sequence map of the LAMB1-Dendra2 HDR template donor, with gRNA protospacer adjacent motifs sites highlighted in pink and linker sequence in yellow. c Gel image from PCR performed on DNA from transfected cells and using primers designed to amplify only when the Dendra2 sequences was inserted in the appropriate genetic location. d Transfected cells fixed with a DAPI counterstain were imaged by confocal analysis. Scale bars represent 20 μm

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