Fig. 1

Immunofluorescent staining of PD-1, PD-L1 and CD3 in AITL. a Immunofluorescent double staining of PD-1 (Cy3, red) and PD-L1 (Alexa Fluor-488, green). PD-1 and PD-L1 are expressed poles apart from each other. b Immunofluorescent double staining of PD-1 (Cy3, red) and CD3 (Alexa Fluor-488, green). The majority of CD3-positive cells bear the PD-1 marker. Nuclei counterstained with DAPI (blue channel)