Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects

Table 1 Correlation between the cutoff points and the diagnostic performance (i.e., sensitivity, specificity, and accuracy) of the protein A/G-based and anti-human IgG antibody-based ELISAs for detection of the anti-P. insidiosum antibodies in serum samples

Cutoff point^a	Protein A/G-based ELISA				Anti-human IgG antibody-based ELISA
Cutoff point^a	Cutoff value (EV)^b	Sensitivity^c	Specificity^d	Accuracy^e	Cutoff value (EV)	Sensitivity	Specificity	Accuracy
Mean EV + 5SDs	13.4^f	100.0	100.0	100.0	18.6	80.0	100.0	93.3
Mean EV + 4SDs	11.3	100.0	98.0	98.7	15.4^g	84.0	100.0	94.7
Mean EV + 3SDs	9.3	100.0	98.0	98.7	12.2	84.0	100.0	94.7
Mean EV + 2SDs	7.2	100.0	96.0	97.3	9.0	84.0	98.0	93.3

^aCutoff point = mean EV of the control sera plus 2, 3, 4 or 5 SDs
^bELISA value (EV) = OD value of a serum sample/OD value of the negative control serum
^cSensitivity (%) = true positive samples/(true positive + false negative samples) × 100
^dSpecificity (%) = true negative samples/(true negative + false positive samples) × 100
^eAccuracy (%) = (true positive + true negative samples)/all samples × 100
^fOptimal cutoff point of the protein A/G-based ELISA
^gOptimal cutoff point of the anti-human IgG antibody-based ELISA

ISSN: 1756-0500