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Fig. 1 | BMC Research Notes

Fig. 1

From: Histone deacetylase inhibitors induce expression of chromosomally tagged variant-specific surface protein genes in Giardia lamblia

Fig. 1

Tagging of a chromosomal copy of vsp gene with 3XHA epitope using endogenous tagging method. a The plasmid construct pKS-BSR-vsptr-3XHA containing blasticidin resistance gene (BSR, highlighted in blue), truncated vsp gene (vsptr, highlighted in red), and three copies of hemagglutinin epitope (3XHA, highlighted in green) were linearized with the restriction enzyme Eco721 and then integrated into Giardia genome by homologous recombination. b The forward and reverse primers used for amplifying the full length vsp gene tagged with 3XHA are indicated by arrows. c Agarose gel electrophoresis of DNA size maker (lane 1) and the amplicons representing the full length vsp1267 gene tagged with 3XHA in Glvsp1267-3XHA cell lines (lane 2). d Agarose gel electrophoresis of DNA size maker (lane 1) and the amplicons representing the full length vsp910BA gene tagged with 3XHA in Glvsp9B10A-3XHA cell lines (lane 2)

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BMC Research Notes

ISSN: 1756-0500

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