Fig. 3
From: MDC1 depletion promotes cisplatin induced cell death in cervical cancer cells
Cisplatin induced cytotoxicity results in higher percentage of cells undergoing apoptosis in MDC1 silenced cell lines. a Annexin V and propidium iodide labelling of cells was done after exposure to cisplatin (10 μM) for 24 h and analysis was done using flow cytometry software. b Graphical analysis of the apoptosis data using GraphPad Prism 5.0 software. c Western blot was performed to check p53 Ser 15 status in the cell lines after exposure to Cisplatin (10 µM) for 24 h. Beta actin was taken as the loading control