Evaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures

Table 2 Comparation of carbapenemase, CTX-M and VanA detecting assays

Test	No	Sens	Spec	Source	Methods	Genes	Turnaround time	References
Entero-DR assay	156	100	92–100	Bacterial colonies	Real-time multiplex PCR	bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-like	2 h	Present study
KPC	72	100	100
NDM	28	100	92.2
VIM	19	100	99.3
CTX-M	51	100	92.4
vanA	25	100	100
IMP	4	(4/4)	99.4
OXA-48-like	2	(2/2)	100
	982	100	98.2	Rectal swaps	Real-time multiplex PCR	bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-like	2 h	[12]
Xpert Carba R	206	95	98.1	Rectal swaps	Real-time multiplex PCR	bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-like	1 h	[18]
KPC	120	94.9	99.6
NDM	61	100	99
VIM	10	–	99.8
IMP	9	100	99.8
OXA-48-like	6	100	99.9
CARBA-5 NG	152	88.2	100		Immunochromatography	bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-like	15 min	[19]
KPC	13	100	100
NDM	29	96.6	100
VIM	48	100	100
IMP	9	55.6	100
OXA-48-like	40	100	100
BD-MAX CPO	175	97.1	98.8	Bacterial colonies and rectal swaps	Real-time multiplex PCR	bla_KPC, bla_VIM, bla_NDM, bla_IMP and bla_OXA-48-like	1 h	[20]
BD MAX ESBL screen	354	95.2	98.8	Rectal swaps	Real-time multiplex PCR	bla_CTX-M-1, bla_CTX-M-2, bla_CTX-M-9 and bla_SHV	1 h	[21]
Filmarray BCID panel
KPC	25	100	100	Blood	Real-time multiplex PCR	bla_KPC, mecA, vanA/B	1 h	[22, 23]
vanA/B	31	100	100	Blood	Real-time multiplex PCR	bla_KPC, mecA, vanA/B	1 h	[22, 23]

ISSN: 1756-0500