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Table 1 Trachymyrmex septentrionalis polymorphic microsatellite markers analyzed for Trachymyrmex septentrionalis ants

From: Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

Marker

Primer sequence 5′- > 3′

Repeat motif

Tm (°C)

Size (bp)

N

K

Ho

He

PI

Ts3a,b,c

F: GCGCGGTTGTTCTTTATGG

R: TTGTATTCTTTGTCATACAGTACGTTGC

ATTTT

60.5

264–292

25

12

0.68

0.88

0.03

Ts4b,c

F: CTTTGAAATCGTCATCGCGG

R: ACGCCCACACGTATACCACC

TCGGC

57.3

242–266

25

11

0.56

0.87

0.03

Ts5a,c

F: CCACCTTGGTAACTGTCGCC

R: CTTTGAAATCGTCATCGCGG

TCGGC

60.5

230–256

48

11

0.67

0.81

0.06

Ts11b,c

F: GCAGATACAAACGTCCTACGTGC

R: CGCACATTTGTGACGGACG

TGCG

66.4

274–358

20

13

0.45

0.91

0.02

Ts12b,c

F: ATTCCTGGCACGGATACACG

R: ACTCTATTGTTGCGCACCGC

ATAC

61.3

164–214

26

9

0.35

0.65

0.16

Ts21c

F: CCATCCCAACCATCCTGG

R: TTACGATCAGGAGAGCGTGC

AGCC

65.2

302–326

45

11

0.76

0.82

0.05

Ts25c

F: CGTAAATTAAAGTGCACAGTCCG

R: GATCGCGATTTGGTGATACG

TCTG

61.3

286–346

51

18

0.78

0.86

0.03

Ts32b,c

F: ATAACAAGCGGCAGCATCG

R: ATTTCGAACTCGCCGGTAGC

TTGG

59.4

200–242

49

15

0.76

0.85

0.04

Ts33

F: AATCAAATGCTTGCGTGTGC

R: CCGGTTAGAAGAAACAGGCG

TGCG

60.5

248–296

51

15

0.71

0.73

0.09

Ts34b,c

F: GTGAGGGAATGAGAGGGAGG

R: TGGTAATGATCGGTACATGATGC

TGCG

61.3

246–490

27

12

0.11

0.81

0.06

Ts35b,c

F: TGCTCGATTCGGACACGG

R: CTCACAGCGGAGACAAAGGC

ACCG

60.5

188–300

49

14

0.89

0.76

0.08

Ts36b,c

F: TGTAGGGATTTAGATGCGGC

R: TAGCCAGACCTCGTACTTCG

TGCG

59.4

166–210

13

9

0.15

0.85

0.04

Ts39c

F: CTAACAAGATGCGCAGCCC

R: TCGAATAATCCAGTCGTGTCG

TGCC

61.3

234–350

50

30

0.84

0.94

0.01

Ts41

F: TTAACGTCGGCATAATTTCGG

R: CAATTGACTACGCAGGAGCG

TGCC

61.3

206–236

46

14

0.78

0.82

0.05

Ts43c

F: CGTCTTTATATTGTATTTGCTTGATACGC

R: GTCCATGCACACGTCCAGC

TGCG

61.3

246–358

47

33

0.72

0.94

0.01

Ts45b,c

F: CGTGTCAAGTATGTTCCCGC

R: AGTTTCAGGCGCAGGTAGC

TGCG

61.3

174–316

48

28

0.60

0.89

0.02

Ts46c

F: GTACGCACATCGTGCTAAACG

R: AGCGGTGGTGGTTTCACG

TGCG

60.5

310–348

46

20

0.78

0.92

0.01

  1. Details include: marker information, primer sequences, repeat motif, annealing temperature (Tm), size range of observed alleles given in base pairs (bp), number of individuals genotyped (N), number of alleles observed (K), observed heterozygosity (Ho), expected heterozygosity (He), and probability of identity (PI)
  2. aIndicates that 30 cycles were used in the PCR profile
  3. bIndicates deviation from Hardy–Weinberg expectations after Bonferroni corrections
  4. cIndicates the marker shows evidence of null alleles
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BMC Research Notes

ISSN: 1756-0500

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