A naturally-occurring 22-bp coding deletion in Ugt86Dd reduces nicotine resistance in Drosophila melanogaster

BMC Research Notes

Table 1 Summary of nicotine resistance phenotypes

Genotype	InDel status	Larval assay		Embryo assay
Genotype	InDel status	Diff (N − C)^a	p-value^b	C^c	N^c	p-value^b	Dev. delay (d)^d
A4	Ins	0.01	0.908	0.76	0.72	0.159	0.24
A4-Ugt86Dd^wt	Ins	− 0.07	0.230	0.77	0.83	0.100	0.26
A4-Ugt86Dd^Del1	1-bp Del	− 0.15	0.071	0.67	0.53	0.007	1.26
A4-Ugt86Dd^Del11	11-bp Del	− 0.25	0.001*	0.62	0.58	0.467	0.97
A4-Ugt86Dd^Del22−13H	22-bp Del	− 0.23	< 0.001*	0.69	0.53	< 0.001*	1.24
A4-Ugt86Dd^Del22−17B	22-bp Del	− 0.18	0.003*	0.61	0.44	0.009	1.41
A4-Ugt86Dd^Del22−23C	22-bp Del	− 0.23	< 0.001*	0.56	0.45	0.092	1.49
A4-Ugt86Dd^Del22−26I	22-bp Del	− 0.22	0.014	0.61	0.47	0.002*	1.62
A3	22-bp Del	− 0.79	< 0.001*	0.39	0.02	< 0.001*	3.83

^aThe difference between the fraction of adults emerging from nicotine (N) vials minus the fraction emerging from control (C) vials. Negative values imply that adult emergence is lower in the presence of nicotine. Also see Fig. 2
^bThe result of genotype-by-genotype Welch’s two sample t-tests comparing the per-vial fraction of adults emerging in nicotine and control treatments. Those p-values marked with an * are significant following a per-assay Bonferroni correction for multiple tests (i.e., 0.05/9)
^cThe fraction of adults emerging from embryos in control (C) vials or nicotine (N) vials
^dThe difference (in days, d) between the average emergence day of adults on nicotine media and that on control media. All values are positive, implying that development time is longer under nicotine conditions. All genotypes showed a significant developmental delay on nicotine media (Welch’s t-tests, p < 0.001)

ISSN: 1756-0500