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Fig. 2 | BMC Research Notes

Fig. 2

From: A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma brucei

Fig. 2

Trypanosomes tagged with mNeonGreen at the C-terminus of phosphodiesterase B1 (PDEB1). a Tagging PDEB1 in Lister 427/Cas9. Scale bar: 10 microns. b Tagging PDEB1 in Lister 427. Fluorescence intensity of live cells was quantified with a benchtop flow cytometer (ACEA NovoCyte). To remove particles of subcellular size, a cut-off of 3 x 104 was applied to the forward scatter. A total of 104 events were recorded and analysed using FlowJo software without gating. TF transfection

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