Fig. 3From: A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma bruceiFlow chart of sequential transfections. Procyclic form trypanosomes were transfected first with pAi1C9 to allow expression of Cas9 and T7RNAP. After 20 h, the pool of trypanosomes was transfected a second time to provide the templates for gRNA(s) and the repair template(s)/resistance cassette(s)Back to article page