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Fig. 1 | BMC Research Notes

Fig. 1

From: CaXyn30B from the solventogenic bacterium Clostridium acetobutylicum is a glucuronic acid-dependent endoxylanase

Fig. 1

Homology comparison of CaXyn30B (UniProt Q97TI3) with CaXyn30A (UniProt Q97TI2) from C. acetobutylicum ATCC 824. Gene organization of the predicted operon containing CaXyn30B and the previously described GA-independent endoxylanase, CaXyn30A. GenBank locus tag identifiers are shown in parenthesis, the arrow indicates direction of transcription (top). Depiction of C-terminal bias of homology between these linked genes. While the proteins show high homology overall, the homology is heavily weighted towards the non-specificity determining C-terminal regions (middle). Alignment detail comparisons of the β7-α7/β8-α8 catalytic cleft of representative gram-positive GA-dependent GH30-8 endoxylanases from Bacillus subtilis (BsXynC), CaXyn30B (this study), Clostridium thermocellum (CtXyn30A), and Paenibacillusbarcinonensis BP-23 (PbXyn30D), with the characterized GA-independent GH30-8 endoxylanases from Clostridium papyrosolvens (CpXyn30A), the linked CaXyn30A, and a gram-negative GA-dependent representative from Erwinia chrysanthemi (EcXynA) (bottom). The β8-α8 loop region of CaXyn30B is boxed in red, the corresponding loop region in the GA-independent endoxylanases are boxed in black and the location of the GA coordinating “RR-motif” is boxed in blue and highlighted below. Operon prediction provided by www.microbesonline.org/operons/gnc272562.html

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