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Table 1 Summary of the relative abundance levels of different taxonomic groups

From: Cell density alters bacterial community structure in culture-enriched 16S rRNA gene microbiota profiling

Level

Taxa

T-ZERO (%)

M-LOW (%)

M-MEDIUM (%)

M-HIGH (%)

Phylum

Firmicutes

(82.03 ± 7.52)a

(93.35 ± 3.82)a

(94.91 ± 3.97)a

(91.68 ± 5.84)a

Proteobacteria

(17.97 ± 7.52)a

(6.12 ± 3.33)a

(5.09 ± 3.97)a

(7.98 ± 5.85)a

Genus

Lactic acid bacteria (LAB)

Lactobacillus

(69.01 ± 6.15)a

(81.21 ± 4.48)a

(77.82 ± 5.27)a

(70.01 ± 5.07)a

Enterococcus

(1.24 ± 1.24)b

(5.62 ± 2.31)ab

(12.11 ± 3.66)a

16.37 ± 5.64)a

Pediococcus

(0.00 ± 0.00)b

(0.00 ± 0.00)b

(1.31 ± 0.67)ab

(3.50 ± 1.47)a

Streptococcus

(0.00 ± 0.00)a

(1.78 ± 1.22)a

(0.49 ± 0.49)a

(0.66 ± 0.66)a

Other than LAB

Bacillus

(0.00 ± 0.00)a

(0.59 ± 0.59)a

(0.00 ± 0.00)a

(0.00 ± 0.00)a

Turicibacter

(11.76 ± 1.72)a

(3.11 ± 1.85)b

(1.93 ± 0.99)b

(1.16 ± 0.77)b

Citrobacter

(1.69 ± 1.69)a

(4.03 ± 3.40)a

(4.03 ± 4.03)a

(6.74 ± 5.96)a

Other grouping

Non Lactobacillus

(30.99 ± 6.15)a

(18.79 ± 4.48)a

(22.18 ± 5.27)a

(29.99 ± 5.07)a

LAB other than Lactobacillus*

(1.24 ± 1.24)c

(7.99 ± 2.98)bc

(13.91 ± 4.08)ab

(20.52 ± 5.24)a

Other than LAB

(29.74 ± 6.00)a

(10.80 ± 4.80)b

(8.27 ± 3.90)b

(9.47 ± 5.76)b

Species

L. johnsonii

(0.00 ± 0.00)b

(4.66 ± 1.44)a

(4.56 ± 1.23)a

(3.12 ± 1.39)ab

L. reuteri

(6.76 ± 1.77)a

(1.70 ± 0.84)b

(0.00 ± 0.00)b

(1.00 ± 1.00)b

L. salivarius

(16.38 ± 1.56)b

(18.85 ± 1.74)ab

21.66 ± 2.13)ab

(23.53 ± 3.19)a

L. ultunensis

(0.00 ± 0.00)b

(0.64 ± 0.64)ab

(1.72 ± 0.87)a

(0.54 ± 0.54)ab

  1. Values are presented in mean ± SEM (Standard Errors of Means). Different letters across each row show statistically significance at p < 0.05 (ANOVA, Student t test). L. acidophilus only present on M-HIGH, absent in all other groups (0.33 ± 0.33)  %. Other species didn’t show any significant differences among different groups