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Table 1 Extraction kits utilized for each product

From: Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes

Species

Product

Extraction kit

Ent PCR

vanA

vanB

Bovine

1

d

+

–

–

2

c

+

–

–

3

d

+

–

–

4

e

+

–

–

5

a

+

–

–

6

a

+

–

–

7

e

+

–

–

Camelid

8

b

–

–

–

Canine

9

b

+

–

–

10

a

+

–

–

11

b

+

–

–

12

a

+

–

–

13

d

+

–

–

14

c

+

+

–

15

a

+

–

–

16

a

+

–

–

17

a

+

–

–

Caprine

18

d

+

+

–

Equine

19

d

+

–

–

20

d

+

–

–

21

d

+

–

–

22

c

–

–

–

23

d

–

–

–

24

d

+

–

–

25

d

+

–

–

26

d

+

–

–

27

d

+

–

–

28

e

+

–

–

29

d

+

–

–

30

e

+

–

–

31

c

–

–

–

Multiple*

32

c

+

–

–

Feline

33

d

+

–

–

34

a

+

–

–

35

d

+

–

–

36

a

+

–

–

37

a

+

–

–

38

b

+

–

–

39

b

+

–

–

40

a

+

–

–

  1. Letters under the extraction kit column signify the QIAGEN ® (Valencia, CA USA) extraction kit utilized: (a) DNeasy PowerFood Microbial Kit, (b) DNeasy PowerSoil Kit, (c) DNeasy Blood and Tissue Kit, (d) QIAamp DNA Stool Mini Kit, (e) DNeasy PowerMax Soil Kit. PCR’s marked as + were identified in that product PCR and for vanA and vanB the PCR product was confirmed to match the gene of interest by sequencing. Ent is indicative of Enterococcus spp.
  2. *Product marketed for use in multiple animal species including horses, pigs, poultry and ruminants