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Fig. 1 | BMC Research Notes

Fig. 1

From: Levobupivacaine inhibits proliferation and promotes apoptosis of breast cancer cells by suppressing the PI3K/Akt/mTOR signalling pathway

Fig. 1

Levobupivacaine inhibits proliferation in breast cancer cells. MCF-7 and MDA-MB-231 cells were treated with different concentrations of levobupivacaine. a Cell viability was measured by CCK-8 assay. IC50 results of levobupivacaine on MCF-7 and MDA-MB 231 cells. b, c Colony formation of MCF-7 and MDA-MB 231 cells treated with various concentrations of Levobupivacaine and stained with crystal violet. d, e The mRNA expression levels of p21 and GAPDH were analysed by qPCR. f, g Protein expression assessment of MCF-7 and MDA-MB-231 cells by western blot against antibodies FOXO1, p21, Cyclin D1 and GAPDH used as control. The data was statistically significant at * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 compared with untreated cells. This data corresponds to the mean ± SEM of three independent experiments

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BMC Research Notes

ISSN: 1756-0500

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