Fig. 2

NAFLD was induced by high-fat emulsion diet. a Macroscopic observation of the liver of the HF+CS group showed a markedly reduced size and paler color compared with the HF group. b Representative images of hematoxylin–eosin and c Masson Trichrome stained sections of liver tissue in different groups at the end of treatments with 100X magnification. A: accumulation of RBCs; I: inflammation; S: steatosis; Arrows in c row: fibrosis. d Histology assessments in control and experimental groups. Values expressed as mean ± SD for 7 rats. *p < 0.05, **p < 0.01, ***p < 0.001, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001; * and ^# symbols respectively indicate comparison to NC and HF groups.NC: normal control diet group; HF: high-fat emulsion group; HF+CS: HF + Capparis spinosa group