Isolation and whole-genome sequencing of Pseudomonas sp. RIT 623, a slow-growing bacterium endowed with antibiotic properties

Table 1 Summary of antiSMASH 5.0 results for Pseudomonas sp. RIT 623

Region	Type of metabolite	From	To	Most similar known cluster	Similarity
2	NAGGN	42,676	57,575	–	–
6	NRPS-like	33,465	75,141	–	–
6	PpyS-KS	186,487	197,805	Pseudopyronine A/pseudopyronine B	43%
15	NRPS	66,508	107,419	Pyoverdine	11%
27	Bacteriocin	48,531	59,358
37	NRPS	1	37,589	Sessilin	55%
47	NRPS	1	30,407	Pyoverdine	6%
60	NRPS-like	1	15,414	Mangotoxin	71%
62	NRPS	1	14,591	Pyoverdine	9%
63	NRPS	1	14,095	Putisolvin	62%
64	NRPS	1	13,527	Pyoverdine	3%
68	NRPS	1	9118	Rhizomide A/rhizomide B/rhizomide C	100%
71	NRPS	1	8037	Rhizomide A/rhizomide B/rhizomide C	100%
81	NRPS-like	1	1419	Xenotetrapeptide	100%

The analysis detected 81 regions of the genome as potential metabolic gene clusters, out of which only 13 contain gene clusters related to antibiotics. The numbering of the nucleotides corresponds to the location of each gene cluster in that particular region (and does not correspond to the coordinates published for the whole genome sequence). The gene clusters with ≥ 50% similarity to known biosynthetic gene clusters are shown in bold
NAGGN N-γ-acetylglutaminyl glutamine 1-amide, NRPS non-ribosomal peptide synthases, PpyS-KS PPY-like pyrone cluster

ISSN: 1756-0500