Fig. 2

Time (pass)-dependent effects of cocaine on BSR in the ICSS test. Upper and lower panels show data of  % BSR thresholds (a) and data of  % maximum wheel-spinning rates (b), respectively, according to the four post-drug treatment passes or time points (15, 30, 45 and 60 min). As described in the figure legend of Fig. 1 and following the same seven-pass ICSS paradigm, cocaine (5 mg/kg) or vehicle (saline) was injected subcutaneously at the end of pass 3 (the time point 0 min). The data of post-treatment BSR thresholds and maximum rates data at each of the four time points were normalized to pre-treatment baselines and presented as  % BSR threshold and  % maximum rate, respectively. Six mice were used for each experimental group. Data were presented as (mean ± SE) and analyzed by Repeated Measure two-way ANOVA with Bonferroni’s Multiple Comparison post hoc tests (*** p < 0.001)