Fig. 2

a Schematic representation of cell culturing method designed to allow matched comparison between primary human dermal fibroblasts subjected to cyclic short-duration strain (CSDS) or CSDS combined with acyclic long-duration strain (ALDS). All conditioned media was collected simultaneously 24 or 96 h after the onset of the CSDS strain profile. b, c Multi-analyte cytokine membrane array analyses on conditioned media collected 24 h (b) or 96 h (c) after the onset of CSDS strain profile. Red boxes indicate the membrane region corresponding to macrophage migration inhibitory factor (MIF), Serpin E1/Plasminogen activator inhibitor (PAI-1), interleukin (IL)-6, IL-8, and chemokine (C-X-C motif) ligand 1 (CXCL1)/Growth-regulated oncogene (GRO)-α. d–g Quantification of cytokine membrane array analyses for MIF (D), Serpin E1 (E), CXCL1 (F), and IL-8 (G). Data are mean ± SEM normalized to CSDS; n = 4 per condition. * indicates p < 0.05 against CSDS by paired t-test. h, i Quantification of cytometric bead array analyses for IL-8 (H) and IL-6 (I). Data are mean ± SEM normalized to CSDS; n = 3 per condition. * indicates p < 0.05 against CSDS by paired t-test