Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

Table 1 Effect of hypocotyl section (proximal or distal), supplements, and growth regulators on the total number of shoots, average shoots per explant, percentage of callus, and percentage of elongated shoots of > 2.5 cm of Plukenetia volubilis hypocotyls after 45 d of cultivation

Components	Hormonal treatments	Average of shoots per explant		Percentage of callus		Percentage of elongated shoots (> 2.5 cm)
Components	Hormonal treatments	Proximal	Distal	Proximal	Distal	Proximal	Distal
CuSO₄	TDZ (1 mg/L)	3.04^ab	1.91^bc	68.89^a	72.78^a	46.74^a	29.63^a
	TDZ (1 mg/L) + 2ip (0.5 mg/L)	3.11^ab	1.73^ab	73.33^a	75.0^a	45.81^a	25.5^a
	TDZ (1 mg/L) + Zeatin (0.5 mg/L)	4.62^d	2.31^c	72.22^a	74.44^a	51.74^ab	37.96^a
	TDZ (1 mg/L) + BAP (0.5 mg/L)	3.93^cd	1.53^ab	76.11^a	77.78^a	48.54^a	26.67^a
	TDZ (1 mg/L) + Kinetin (0.5 mg/L)	3.51^bc	1.62^ab	75.56^a	78.33^a	33.19^a	28.52^a
	TDZ (1 mg/L) + IBA (0.5 mg/L)	3.42^abc	1.47^ab	75^a	75.56^a	41.33^a	26.67^a
	TDZ (1 mg/L) + NAA (0.5 mg/L)	3.36^abc	1.8^b	76.11^a	79.44^a	40.70^a	27.04^a
	Control	2.71^a	1.27 ^a	13.33^b	11.11^b	69.26^b	68.52^b
G + A + A	TDZ (1 mg/L)	3.33^abc	1.76^bc	70^b	73.33^a	41.04^ab	30.37^a
	TDZ (1 mg/L) + 2ip (0.5 mg/L)	3.29^ab	1.8^bc	73.33^bc	72.33^a	44.56^ab	27.41^a
	TDZ (1 mg/L) + Zeatin (0.5 mg/L)	5.11^e	2.2^c	73.89^bc	75.0^a	53.34^b	36.67^a
	TDZ (1 mg/L) + BAP (0.5 mg/L)	4.87^de	1.62^b	76.11^bc	77.78^a	45.69^ab	29.63^a
	TDZ (1 mg/L) + Kinetin (0.5 mg/L)	4.11^cd	1.51^ab	76.07^bc	76.67^a	31.51^a	27.41^a
	TDZ (1 mg/L) + IBA (0.5 mg/L)	3.71^abc	1.6^b	78.33^bc	76.11^a	45.70^ab	29.63^a
	TDZ (1 mg/L) + NAA (0.5 mg/L)	3.93 ^bc	1.73 ^bc	78.89 ^c	80.0^a	38.56 ^ab	28.15 ^a
	Control	3.0^a	1.09^a	14.44^a	11.11^b	77.26^c	65.93^b

ISSN: 1756-0500