Fig. 2

Characterization of Z. mobilis strains that generated from serial co-culture-based laboratory adaptation. a Total production of acetate, lactate and ethanol from the overnight monoculture of the generated Z. mobilis strains and the parental strain Zm6. b Growth profiles of monoculture Z. mobilis strain obtained in this study and the parental strain Zm6 in the complex medium with glucose 20 g/L. Z. mobilis strain designations are as follows; Zs100; Z. mobilis strain obtained from the last round of Z. mobilis vs S. cerevisiae serial co-culture with glucose 100 g/L, Zs100R; obtained from a parallel replicate of Z. mobilis vs S. cerevisiae glucose 100 g/L, Zs20; Z. mobilis strain obtained from the last round of serial co-culture with S. cerevisiae supplemented with 20 g/L glucose, Zs20R; Z. mobilis strain from a last culture of parallel run of Zs20, Ze20; Z. mobilis strain obtained from last round of Z. mobilis vs E. coli co-culture with 20 g/L glucose, and Ze20R as a parallel replicate of Ze20, Z20 designates Z. mobilis strain obtained from a last round of Z. mobilis monoculture with 20 g/L glucose, and Z100 designates Z. mobilis strain obtained from a last round of monoculture with 100 g/L glucose. Generation time for actively growing Z. mobilis strains in the plate reader was as follows; 175 min for Zm6, 177 min for Zs20, 173 min for Zs100 and 179 min for Ze20. Note that there were no significant differences in ethanol production and growth profiles between strains. Error bars; standard deviations from 3 independent measurements