TY - JOUR AU - Dunislawska, Aleksandra AU - Szczerba, Agata AU - Siwek, Maria AU - Bednarczyk, Marek PY - 2020 DA - 2020/09/18 TI - Dynamics of the transcriptome during chicken embryo development based on primordial germ cells JO - BMC Research Notes SP - 441 VL - 13 IS - 1 AB - Regulation of gene expression during embryo development on the basis of migration of primordial germ cells (PGCs) in vivo has been rarely studied due to limited cell number and the necessity to isolate PGCs from a large number of embryos. Moreover, little is known about the comprehensive dynamics of the transcriptome in chicken PGCs during early developmental stages. The current study investigated transcriptome dynamics of chicken PGCs at key developmental stages: 4.5, 8 and 12 days of embryo incubation. PGCs were collected, and RNA was isolated using a commercial kit for single cells. The isolated RNA was subjected to microarray analysis (Agilent Technologies). SN - 1756-0500 UR - https://doi.org/10.1186/s13104-020-05286-w DO - 10.1186/s13104-020-05286-w ID - Dunislawska2020 ER -