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Table 2 Expected and observed results of PCR and LAMP diagnostic marker for MS1 in Cryptomeria japonica

From: Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

Maker ID

Expected size (bp)#1 or presence/absence (±) of products

Observed size (bp) or presence/absence (±) of products

Ms1 (wild allele)

ms1-1 (4-bp deletion)

ms1-2 (30-bp deletion)

Ms1 (wild allele)

ms1-1 (4-bp deletion)

ms1-2 (30-bp deletion)

ALP_ms1-1

165

161

165

162

158

162

ALP_ms1-2

146

146

116

141

141

112

ALP_ms1

172/288#2

168

258

169/286#2

165

255

ASP_ms1-1

–

103

–

–

+

–

ASP_ms1-1_wt

105

–

105

+

–

+

LAMP_ms1-1

–

+

–

–

+

–

LAMP_ms1-1_wt

+

–

+

+

–

+

LAMP_ms1-2

–

–

+

+#3

+#3

+

  1. #1Tail sequences of primers are included to calculate the expected PCR product size
  2. #2Wild type allele is expected to produce fragments with 172 and 288 bp without 4- and 30-bp deletions, respectively
  3. #3Products are still absent after 60 min incubation, but present after 120 min incubation (Additional file 6)