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Table 2 Expected and observed results of PCR and LAMP diagnostic marker for MS1 in Cryptomeria japonica

From: Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

Maker ID Expected size (bp)#1 or presence/absence (±) of products Observed size (bp) or presence/absence (±) of products
Ms1 (wild allele) ms1-1 (4-bp deletion) ms1-2 (30-bp deletion) Ms1 (wild allele) ms1-1 (4-bp deletion) ms1-2 (30-bp deletion)
ALP_ms1-1 165 161 165 162 158 162
ALP_ms1-2 146 146 116 141 141 112
ALP_ms1 172/288#2 168 258 169/286#2 165 255
ASP_ms1-1 103 +
ASP_ms1-1_wt 105 105 + +
LAMP_ms1-1 + +
LAMP_ms1-1_wt + + + +
LAMP_ms1-2 + +#3 +#3 +
  1. #1Tail sequences of primers are included to calculate the expected PCR product size
  2. #2Wild type allele is expected to produce fragments with 172 and 288 bp without 4- and 30-bp deletions, respectively
  3. #3Products are still absent after 60 min incubation, but present after 120 min incubation (Additional file 6)