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Fig. 1 | BMC Research Notes

Fig. 1

From: MamA essentiality in Mycobacterium smegmatis is explained by the presence of an apparent cognate restriction endonuclease

Fig. 1

mamA depletion causes growth cessation in M. smegmatis. a Strains with depleted mamA (mamA KD), nonspecific CRISPRi control with a sgRNA sequence that does not target any specific part of M. smegmatis genome (Nonspecific sgRNA), or a rescue vector containing a synonymous mamA mutant that is not bound by dCas9 (mamA Rescue) were incubated in 7H9 in presence (+) or absence of ATc to activate or not the CRISPRi knockdown system, respectively. Three biological replicates of mamA KD, four biological replicates of mamA Rescue, and two technical replicates of each were used and values were averaged. b The strains were grown in 7H9 and CFUs were calculated at different timepoints

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