Fig. 1
From: In invasion assays, the breast cancer cell nucleus leads the way
a Schematic of the experimental set-up. A 24-well insert of an invasion assay with MDA-MB-231 cells were placed on a shallow plastic ring into a 1-well chamber slide. This set-up allowed for confocal imaging. b MDA-MB-231 cells stably expressing CapG-eGFP imaged with epifluorescence in an invasion assay. I: Cancer cells not invading but remaining on top of the porous membrane are spindle shaped. Figure II-IV: Cancer cells that did invade the pores of an invasion assay showed a change in morphology and assumed an amoeboid-like cell shape. Scale bar 10 um