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Table 2 Summary of findings

From: In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts

Analysis Week 0 (fresh) Week 1 (frozen) Week 4 (frozen) Week 8 (frozen) Week 31 (frozen) Conclusions
Nicotine (µg/ml) 8268 ± 635.15 7980 7250 8310 9110 No effect up to 31 weeks storage at – 80 °C
OD at 260 nm 2.26 ± 0.09 2.26 2.30 2.29 2.23 No effect up to 31 weeks storage at – 80 °C
OD at 320 nm 0.57 ± 0.02 0.57 0.58 0.57 0.58 No effect up to 31 weeks storage at – 80 °C
H292 cell viability (%; measured at 50% toxicity dose [56% AqE]) 54.48 ± 4.0 56.6 ± 4.50 60.4 ± 3.3 56.9 ± 8.3 64.0 ± 2.9 No significant effect up to 31 weeks storage at – 80 °C (p = 0.138 for difference between weeks 0 and 31)
Glutathione ratio
(measured at 50% toxicity dose [56% AqE])
60.25 ± 2.8 43.90 ± 5.49 43.15 ± 7.61 55.08 ± 4.25 54.47 ± 6.42 No significant effect up to 31 weeks storage at – 80 °C (p = 0.548 for difference between weeks 0 and 31)
  1. Data are means and standard deviations (where available)
  2. AqE, aqueous aerosol extracts; H292, Human bronchial epithelial cells (NCI-H292; American Type Culture Collection, Teddington, Middlesex, UK); OD, optical density