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Fig. 2 | BMC Research Notes

Fig. 2

From: Performance characteristics of a quantitative PCR assay on repository stool specimens and smeared filter-paper cards

Fig. 2

FTA card and stool samples positive vs. samples negative on microbiologic testing (n = 109). ETEC detection by TAC includes multiple targets: LT, STh and STp. Norovirus detection by TAC on FTA cards and stool includes the Norovirus GI or Norovirus GII target. Shigella and Campylobacter detection was based on a single target. Significant difference in median Cq values observed for the following by pairwise comparison: Campylobacter: Benchmark (+)/FTA Card(+) vs. Benchmark(−)/FTAcard(+) p = 0.036. Shigella/EIEC: Benchmark (+)/FTA Card(+) vs. Benchmark (−)/FTAcard(+) p = 0.046. Norovirus: Benchmark (−)/Stool (+) N = 18 Benchmark (-)/FTAcard(+) p = 0.003. Benchmark (+)/Stool (+) N = 7 Benchmark (+)/FTAcard(+) p = 0.033. The correlation between corresponding stool and FTA card targets was poor (r2 : ETEC 0.37, Campylobacter: 0.27, Shigella 0.40 and Norovirus 0.07; p < 0.01 for all)

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