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Fig. 1 | BMC Research Notes

Fig. 1

From: The presence of membrane bound CD99 ligands on leukocyte surface

Fig. 1

Identification of CD99 ligands by pull-down method with DTSSP cross-linker. THP-1 cells were stained with CD99HIgG or CD147ExHIgG fusion proteins and crosslinked with DTSSP. Following cell lysis, the complexes of CD99HIgG with their ligands were pulled-down using protein G beads. a CD99HIgG or b CD147ExHIgG was used as bait protein for pull-down experiments. Western blotting under non-reducing conditions was performed using anti-CD99 mAb (MT99/3) or anti-CD147 mAb (MEM-M6/3), followed by HRP conjugated rabbit anti-mouse immunoglobulins antibodies. Lane 1; CD99HIgG or CD147ExHIgG recombinant proteins used in the pull-down method. Lane 2; pull-down material obtained via Protein A beads. Lane 3; pre-pull-down represents the material before the pull-down process. Lane 4; flow through represents the material obtained following the pull-down process. The molecular weights (kDa) of protein markers are displayed on the left

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