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Fig. 3 | BMC Research Notes

Fig. 3

From: Longitudinal assessment and stability of long non-coding RNA gene expression profiles measured in human peripheral whole blood collected into PAXgene blood RNA tubes

Fig. 3

Long term storage and freeze–thaw cycles do not alter lncRNA or mRNA expression. qRT-PCR gene expression data were produced for a six lncRNAs and b six mRNAs. Baseline RNA immediately isolated from PAXgene Blood RNA tubes was compared to three treatment conditions including RNA storage at − 80 °C for one year, total RNA from PAXgene tubes exposed to five freeze–thaw cycles, and total RNA that was exposed to ten freeze–thaw cycles. Paired sample t-test was used to determine statistical significance. Bars represent mean Log2(ΔΔCt) with SD for n = 5 individual healthy subject’s baseline total RNA samples and after one of the three treatment conditions

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