Skip to main content
Fig. 1 | BMC Research Notes

Fig. 1

From: Toward the identification of a phytocannabinoid-like compound in the flowers of a South African medicinal plant (Leonotis leonurus)

Fig. 1

HPTLC phytochemical profile of Leonotis leonurus var. albiflora Benth. whole flower extract. a L. leonurus var. albiflora Benth. white flower phenotypic appearance (Photo courtesy of Dr B Loedolff) and b its corresponding acetonitrile (75%) extract, HPTLC phytochemical profile. Whole flower metabolite extractions were prepared using various extraction solvents and, the acetonitrile (75%) extracts were selected for downstream analyses. The phytochemical profile was developed fully (mobile phase; chloroform:methanol 9:1, stationary phase; silica60Å) before reagent visualization (0.1% Fast blue B salt dissolved in 1 mM NaOH) was applied to reveal potential pCB candidates, based solely on an red–orange colour development. Compound x (Rf value: 0.55) represents a colorimetric-specific pCB-like candidate compound which was selected for further LC–MS/MS analyses

Back to article page