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Table 3 PCR condition for bcsp31 template

From: Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species

Feature Temperature (°C) Time
Gene (bcsp31)
 Initial denaturation 95 5 min
 Denaturation 95 60 s
 Annealing 65 30 s
 Extension 72 60 s
 Final extension 72 6 min
 Cycle 35