Fig. 1

ADAM9 was not regulated by Merlin in VS. a Western-blot of ADAM9 expression in four representative VS of n = 24. b Merlin protein expression in VS primary cells after lentiviral transfection. The immunofluorescent images compare successfully transfected green fluorescent cells (left) with the same field of view showing all cells (right) to estimate the transfection rate. c Western-blot of MCF7 breast cancer cell-lysate (MCF7), suggested by the manufacturer of the Merlin antibody as positive control, VS primary cells transfected with Merlin (Merlin) and untransfected VS primary cells (Control). Cell lysates were loaded twice onto the same gel to avoid stripping of the blot, which then was cut into half for the incubation with Merlin and ADAM9 antibody, respectively. Shown is one representative experiment of n = 3. γ-Tubulin served as loading control in all Western-blot experiments. Blots were cropped for better clarity. The full length blots are presented in Additional file 1, Fig. S1 and full length blots of the two additional experiments in Additional file 1, Fig. S2a. d Quantification of ADAM9 expression in Merlin overexpressing (Merlin) and untransfected VS primary cells (Control) as analyzed in (c)