Fig. 3
From: Air sampling for detection of infectious laryngotracheitis (ILT) in commercial poultry flocks
Identification of gallid herpesvirus 1 from feather shafts by PCR. Pools of feathers (n = 4) per sample were collected randomly from the floor of the poultry shed and presence of gallid herpesvirus 1 was determined by PCR. A single band is shown that corresponds to predicted amplicon containing the TK gene (2250 bp). The purified gallid herpesvirus 1 DNA from infected tissue was used as a positive control (+) template, and no amplification was seen in the negative (−), no template, control