Skip to main content

Table 1 Introduction of CRISPR/Cas9 system into embryos at different stages by cytoplasmic microinjection and electroporation

From: Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

Embryonic stage Gene editing method Cas9/gRNAcomplex* No. of embryos examined No. (%) of embryos
Cleavage** Developed to blastocysts
1-cell MI  −  201 138 (68.7 ± 2.6)a 21 (10.5 ± 1.7)a
 +  248 155 (62.4 ± 2.9)a 20 ( 8.1 ± 1.1)a
EP  −  210 192 (91.5 ± 1.7)b 38 (18.1 ± 1.4)ab
 +  250 230 (92.0 ± 0.9)b 40 (16.0 ± 3.1)ab
2-cells MI  −  105 78 (74.3 ± 1.7)a 19 (18.2 ± 1.8)ab
 +  119 73 (61.5 ± 6.2)a 15 (13.4 ± 6.4)a
EP  −  105 91 (86.9 ± 1.9)b 34 (32.6 ± 1.9)c
 +  128 114 (89.7 ± 4.3)b 33 (26.3 ± 7.7)bc
  1. MI microinjection, EP electroporation
  2. All of the experiments were repeated four to five times. Data are expressed as the mean ± SEM
  3. * The embryos at the 1-cell and 2-cell stages were microinjected and electroporated with (+) or without (−) gRNA and Cas9 complexes
  4. ** The embryos treated at 2-cell stage were defined as cleavage when they have more than 3 blastomeres on day 7
  5. a−cValues with different superscripts in the same column are significantly different (p < 0.05)