Table 1 Comparison of the TCA/Acetone, TCA/Acetone with dialysis and, Clean-up Kit methods with dialysis for protein precipitation of hydatid cyst fluid (HCF) based on their outlay and quality results
Methods | Laboratory materials and facilities | Outlay | Quality | Outcome |
|---|---|---|---|---|
TCA/Acetone | Acetone, TCA, DTT | Low | Poor | A lot of smears and indiscernible protein spots |
TCA/Acetone + dialysis | Acetone, TCA, DTT 3 kDa centrifugal filter (Ultra-0.5) | Middle | Good | Fewer smears and separated protein spots |
2-D Clean-Up Kit + dialysis | 2-D Clean-Up Kit 3 kDa centrifugal filter (Ultra-0.5) | High | Best | Without smears and sharp and clear protein spots |