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Table 1 Comparison of the TCA/Acetone, TCA/Acetone with dialysis and, Clean-up Kit methods with dialysis for protein precipitation of hydatid cyst fluid (HCF) based on their outlay and quality results

From: Proteomic characterization of hydatid cyst fluid: two-dimensional electrophoresis (2-DE) setup through optimizing protein extraction

Methods Laboratory materials and facilities Outlay Quality Outcome
TCA/Acetone Acetone, TCA, DTT Low Poor A lot of smears and indiscernible protein spots
TCA/Acetone + dialysis Acetone, TCA, DTT
3 kDa centrifugal filter (Ultra-0.5)
Middle Good Fewer smears and separated protein spots
2-D Clean-Up Kit + dialysis 2-D Clean-Up Kit
3 kDa centrifugal filter (Ultra-0.5)
High Best Without smears and sharp and clear protein spots
  1. TCA trichloracetic acid, DDT dithiothreitol