Fig. 1
From: Morphological study of embryonic Chd8+/− mouse brains using light-sheet microscopy
Volumetric analysis of CUBIC-cleared wild-type and Chd8+/– samples, immunostained for N-cadherin (red) to mark neuronal epithelial tissue. a Illustration of the processing steps in the creation of manual surfaces. Sequential ventricular contours were drawn manually throughout the entire dataset to extract 3D morphology (white outlines). Scale bar 500 µm. b Raw (top row) and overlays (bottom row) of ventricular (yellow) and cortical (blue) iso-surfaces for each sample. Scale bar 400 µm