Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid

Table 1 Comparison of Gram and AO stain in the direct microscopic examination of CSF specimens (n = 387)

Gram stained smear microscopy results versus culture results
Gram’s stain	Culture		Sensitivity	Specificity	Predictive value
Gram’s stain	Pos	Neg	Sensitivity	Specificity	Pos	Neg
Pos	25	0	78.12%	100%	100%	98.06%
Neg	7	355	78.12%	100%	100%	98.06%

AO stained smear microscopy results versus culture results
AO stain	Culture		Sensitivity	Specificity	Predictive value
AO stain	Pos	Neg	Sensitivity	Specificity	Pos	Neg
Pos	31	4	96.88%	98.87%	88.57%	99.70%
Neg	1	351	96.88%	98.87%	88.57%	99.70%

ISSN: 1756-0500