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Table 1 Size of barcode sequences obtained for 48 plants from 16 different species of forage grasses and legumes

From: DNA barcode trnH-psbA is a promising candidate for efficient identification of forage legumes and grasses

BOLD Process IDSpeciesCultivarSequence size in bp [number of n’s in sequence]
rbcLamatKtrnH-psbA
SWFRG013-19Alopecurus pratensis‘Alko’ (Saatszucht Steinach, DE)0a838 [0]0a
SWFRG014-19Alopecurus pratensis‘Alopex’ (Agroscope, CH)0a663 [0]0a
SWFRG029-19Alopecurus pratensis‘Alopex’ (Agroscope, CH)552 [0]185 [0]569 [0]
SWFRG015-19Arrhenatherum elatius‘Arone’ (Saatszucht Steinach, DE)549 [0]436 [0]512 [0]
SWFRG016-19Arrhenatherum elatius‘Median’ (DLF Životice, CZ)550 [0]610 [0]0a
SWFRG031-19Arrhenatherum elatius‘Median’ (DLF Životice, CZ)0a825 [0]0a
SWFRG030-19Cynosurus cristatus‘Cresta’ (Agroscope, CH)541 [0]513 [0]466 [0]
SWFRG045-19Cynosurus cristatus‘Lena’ (HBLF, AT)585 [0]531 [0]564 [0]
SWFRG046-19Cynosurus cristatus‘Rožnovská’ (OSEVA PRO, CZ)529 [0]870 [0]569 [0]
SWFRG001-19Dactylis glomerata‘Barexcel’ (Barenbrug, NL)577 [0]640 [0]519 [0]
SWFRG002-19Dactylis glomerata‘Brennus’ (R2n, FR)546 [0]865 [13]576 [0]
SWFRG017-19Dactylis glomerata‘Reda’ (Agroscope, CH)534 [0]866 [13]561 [0]
SWFRG003-19Festuca pratensis‘Cosmolit’ (Saatszucht Steinach, DE)547 [0]579 [0]558 [0]
SWFRG004-19Festuca pratensis‘Paradisia’ (Agroscope, CH)549 [0]888 [7]566 [0]
SWFRG019-19Festuca pratensis‘Pradel’ (Agroscope, CH)552 [0]590 [0]553 [0]
SWFRG007-19Festuca rubra‘Echo’ (DLF-Trifolium, DK)559 [0]886 [3]274 [21]
SWFRG008-19Festuca rubra‘Pran Solas’ (Schweizer, CH)588 [0]869 [0]570 [0]
SWFRG023-19Festuca rubra‘Roland’ (Saatszucht Steinach, DE)558 [0]543 [0]594 [0]
SWFRG005-19Lolium multiflorum‘Axis’ (Agroscope, CH)581 [0]874 [0]551 [0]
SWFRG006-19Lolium multiflorum‘Caribu’ (Agroscope, CH)577 [0]884 [8]567 [7]
SWFRG021-19Lolium multiflorum‘Zebra’ (Agroscope, CH)571 [0]586 [0]551 [0]
SWFRG009-19Lolium perenne‘Arara’ (Agroscope, CH)547 [0]883 [3]539 [5]
SWFRG010-19Lolium perenne‘Arvella’ (Agroscope, CH)582 [0]481 [0]567 [15]
SWFRG025-19Lolium perenne‘Lipresso’ (Euro Grass, DE)488 [0]835 [0]614 [0]
SWFRG024-19Lotus corniculatus‘Lotar’ (OSEVA UNI, SK)544 [0]399 [0]294 [0]
SWFRG039-19Lotus corniculatus‘Lotar’ (OSEVA UNI, SK)548 [0]509 [0]414 [0]
SWFRG040-19Lotus corniculatus‘Polom’ (CVRV, VÚRV, CZ)502 [0]702 [0]412 [0]
SWFRG022-19Medicago sativa‘Artemis’ (Barenbrug, NL)580 [0]410 [0]268 [14]
SWFRG037-19Medicago sativa‘Catera’ (Saatszucht Steinach, DE)526 [0]435 [0]438 [3]
SWFRG038-19Medicago sativa‘Sanditi’ (Barenbrug, NL)548 [0]432 [0]445 [2]
SWFRG028-19Onobrychis viciifolia‘Perdix’ (Agroscope, CH)550 [0]576 [0]289 [0]
SWFRG043-19Onobrychis viciifolia‘Perly’ (Agroscope, CH)582 [0]627 [0]284 [0]
SWFRG044-19Onobrychis viciifolia‘Višňovský’ (Agrogen, CZ)543 [0]694 [10]287 [0]
SWFRG032-19Phleum pratense‘Anjo’ (ILVO, BE)540 [0]0a0a
SWFRG047-19Phleum pratense‘Tiller’ (DLF-Trifolium, DK)576 [0]527 [5]584 [0]
SWFRG048-19Phleum pratense‘Toro’ (CRA-FLC, IT)0a516 [1]0a
SWFRG011-19Poa pratensis‘Likollo’ (DSV, DE)470 [0]865 [0]540 [0]
SWFRG012-19Poa pratensis‘Nixe’ (Saatszucht Steinach, DE)571 [0]868 [0]576 [0]
SWFRG027-19Poa pratensis‘Tommy’ (DLF-Trifolium, DK)0a489 [0]0a
SWFRG020-19Trifolium pratense‘Bonus’ (Selgen, CZ)549 [0]0a410 [0]
SWFRG035-19Trifolium pratense‘Diplomat’ (DSV, DE)564 [0]556 [0]485 [0]
SWFRG036-19Trifolium pratense‘Pavo’ (Agroscope, CH)514 [0]430 [0]496 [0]
SWFRG026-19Trifolium repens‘Beaumont’ (CW 090; Barenbrug, NL)550 [0]419 [0]448 [0]
SWFRG041-19Trifolium repens‘Bombus’ (Agroscope, CH)579 [0]481 [0]471 [0]
SWFRG042-19Trifolium repens‘Hebe’ (Svalöf-Weibull, SE)571 [0]444 [0]447 [0]
SWFRG018-19Trisetum flavescens‘Gunther’ (HBLFA, AT)504 [0]859 [6]570 [0]
SWFRG033-19Trisetum flavescens‘Gunther’ (HBLFA, AT)575 [0]586 [4]571 [0]
SWFRG034-19Trisetum flavescens‘Trisett51’ (Saatszucht Steinach, DE)558 [0]887 [4]568 [0]
 Total sequences 43 (89.58%)46 (95.83%)41 (85.42%)
  1. a Repeatedly unsuccessful PCR