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Fig. 3 | BMC Research Notes

Fig. 3

From: Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils

Fig. 3

The amount of viable/apoptotic neutrophils, oxidative burst and CD11b expression in AP versus FCS cultures. The rate of apoptosis was inversely proportional to the number of viable neutrophils; the difference between viable (plot A) and apoptotic cells (plot B) were significant at 36 h and 60 h but not at 12 h (n = 32). For comparison of the oxidative burst capacity of neutrophils, “oxidative index” were calculated, that is the ratio of mean fluorescence of stimulated neutrophils to mean fluorescence of unstimulated neutrophils (plot C, n = 28). The increased CD11b expression after LPS stimulation (n = 17) in the neutrophils cultured with two different supplements over time were shown in plot D

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