Fig. 1
From: R-spondin 2 mediates neutrophil egress into the alveolar space through increased lung permeability
Validation of RSPO2 deletion. a RSPO2 gene expression in lung homogenates from RSPO2fl/fl;UBC-Cre-ERT2(+), RSPO2fl/fl;UBC-Cre-ERT2(−), and C57BL/6 mice 48 h post-TM treatment. Cre-recombination of the loxP sites yields a 512 bp fragment, whereas the wild type allele yields a non-specific 600 bp fragment. b qPCR analysis of RSPO2 expression in the cultured fibroblasts isolated from the lungs of RSPO2−/− and RSPO2+/+ mice. c, d Quantification of immunocytochemical evidence of RSPO2 expression in fibroblasts isolated from RSPO2−/− and RSPO2+/+ mice. An arrow indicates examples of DAPI/RSPO2-double stained cells. Representative images are shown from RSPO2+/+ mice (n = 3) and RSPO2−/− mice (n = 3) samples. * = A P value of less than 0.05 was considered significant