Staphylococcus aureus pigmentation is not controlled by Hfq

BMC Research Notes

Table 1 Staphylococcus aureus strains, plasmids and primer used for this study

Strain name	Key features	Reference or construction
RN4220	Transformable by DNA from E. coli	[25]
SAPhB5	RN4220 Δhfq::cat	[13]
NCTC8325	Clinical isolate	[26]
SAPhB224	NCTC8325 Δhfq::cat	NCTC8325 + ϕ11(SAPhB5)
NCTC8325-4	NCTC8325 Δϕ11 Δϕ12 Δϕ13	[27]
SAPhB197	NCTC8325-4 Δhfq::cat	NCTC8325-4 + ϕ11(SAPhB5)
RN6390	NCTC 8325-4 ϕ6390	[28]
SAPhB22	RN6390 Δhfq::cat	[13]
HG001	NCTC8325 rsbU repaired	[22]
SAPhB199	HG001 Δhfq::cat	HG001 + ϕ11(SAPhB5)
HG002	NCTC8325 tcaR repaired	[22]
SAPhB201	HG002 Δhfq::cat	HG002 + ϕ11(SAPhB5)
HG003	NCTC8325 rsbU and tcaR repaired	[22]
SAPhB203	HG003 Δhfq::cat	HG003 + ϕ11(SAPhB5)
COL	Methicillin resistant clinical isolate	[29]
SAPhB16	COL Δhfq::cat	[13]
Newman	Clinical isolate	[30]
SAPhB17	Newman Δhfq::cat	[13]
SAPhB142	RN4220 pRMC2	RN4220 + pRMC2
SAPhB248	RN4220 pRMC2Hfq	RN4220 + pRMC2Hfq
SAPhB251	RN4220 pRMC2HfqFLAG	RN4220 + pRMC2HfqFLAG
SAPhB233	HG003 pRMC2	HG003 + pRMC2
SAPhB249	HG003 pRMC2Hfq	HG003 + pRMC2Hfq
SAPhB257	HG003 pRMC2HfqFLAG	HG003 + pRMC2HfqFLAG

Plasmid name	Key features	Reference/construction
pRMC2	Anhydrotetracycline (aTc) inducible promoter P_xyl/tetO	[17]
pRMC2Hfq	hfq inducible expression	See “Methods”
pRMC2FLAG	pRMC2 derivative for translational gene fusions with 3xflag coding sequence	See “Methods”
pRMC2HfqFLAG	hfq::3xflag inducible expression	See “Methods”

Primer name	Sequence	Purpose
39	GGGGTACCATGATTGCAAACGAAAAC	hfq amplification (with a KpnI site)
49	GGGGAATTCTTATTCTTCACTTTCAGTAGATGC	hfq amplification (with an EcoRI site)
856	GGTACCGTTAACAGATCTGAG	pRMC2 amplification
918	GCTTATTTTAATTATACTCTATCAATGATAGAG	pRMC2 and pRMC2FLAG amplifications
858	TCAGATCTGTTAACGGTACCGGAATTAGCTTGCATGGAA	3xflag amplification
919	GATAGAGTATAATTAAAATAAGCGAGCTCGACTACAAAGACCA	3xflag amplification
865	GACTACAAAGACCATGACGG	pRMC2FLAG amplification
939	GATAGAGTATAATTAAAATAAGCGTAAAAGGAGTCCGACAGATGA	hfq amplification for cloning in pRMC2FLAG
940	CCGTCATGGTCTTTGTAGTCTTCTTCACTTTCAGTAGATGCTTG	hfq amplification for cloning in pRMC2FLAG

ISSN: 1756-0500