Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

Table 1 Thirteen novel microsatellite markers developed for use in Somniosus microcephalus (n = 35)

Locus	Sequence (5′—> 3′)	T_A (°C)	Range (bp)	Motif	N_A
Smic1	F: TGCCTAGTAGACGCCCCTAA	52	157–189	CAGA	7
	R: TGTTCCCAGATGTGTGCATT
Smic2	F: GCCTAAGCCACCCTCCTAAT	57	159–167	ACAG	2
	R: CTCCGGCATCTCCACACTAT
Smic4	F: TATTTAGTCCCAGCAGTGCG	55	205–233	TGAC	6
	R: ACTTCGGCGACCATGTTCTA
Smic5^a	F: TGTTTCAGGAATAGGGATGCC	55	224–244	TCAG	4
	R: CAATCATTTATCTTGTGGAGCCA
Smic10	F: ATGCCTATGACACTCCCCTG	52	176–204	GACA	6
	R: ACCTGCCACCCGATTAGTAA
Smic12	F: TGTCCGACCGAAACGTAAAT	52	173–189	AGAC	3
	R: CCCTCAGCAGAACCATTCAT
Smic13	F: CCCATAAACAGCGAATGACC	55	152–164	AGAC	3
	R: GCCTTTGAACCAAGGACAGA
Smic15	F: ATGCTTAGGACGGTTCTGGA	52	196–220	AGAC	6
	R: ATCCCTCATCCTGTGGACTG
Smic16	F: CAGTGACAAACATCCCCAAA	52	220–236	ATAG	5
	R: AAACAGCCTTTCCCCGTCTA
Smic18	F: ACGTAAATACGCCGATGACC	52	212–224	CAGA	4
	R: GGCCATGAACTTATCCTCCA
Smic20	F: TCCGAACTCTTTTGGCTGAC	55	243–259	GACA	4
	R: CGTTCTCAGCTCAGGGATCT
Smic24	F: TCACTGGTCCGTAATCGTCA	55	205–213	GACA	4
	R: CCACATCTTCCGGCTCTAAA
Smic31	F: ATACGCTTATGACCGCTCCG	57	243–259	GACA	3
	R: GTCCAAAACACAGAGCAGGG

Includes: Locus name, forward (F) and reverse (R) primer sequences, annealing temperature (T_A), fragment length range in base pairs, repeat tetrasat motif, total number of alleles (N_A)
^a indicates presence of null alleles

ISSN: 1756-0500