Fig. 2
Calcein Fluorescence in NBT-II cells Before and After Fluid Shear. Panel a shows cells preloaded with CalceinAM, giving green fluorescence of Calcein in the cytoplasm. Panel b shows fluid shear cause some cell membrane to rupture with loss of the green cytoplasmic fluorescence (marked by star). The membrane ruptured cells appear as “ghost” cells inter-dispersed among live cells. Occasionally there are large patches of ruptured cells as shown in the upper inset in Panel b. Ruptured cells can be stained with propidium iodide as shown in the lower inset in Panel b. Bar = 10 μm