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Table 1 Fluid shear effect of NBT-II cells grown in T-25 of various manufacturers

From: Cell membrane rupture: a novel test reveals significant variations among different brands of tissue culture flasks

Manufacturer

Cat #

Lot #

% Fluid shear effect ± sem

Chemglass(CellTreat)

229331

110723-218

10.5 ± 0.5*

Corning (regular)

3056

09815045

14.1 ± 0.6*

Corning (Cell-Bind)

3289

22005005

8.7 ± 0.5

Falcon

353108

5249004

13.6 ± 1.4*

Greiner

690170

08050140

11.3 ± 0.2*

Nunc

136196

136985

20.6 ± 0.3*

SantaCruz

SC-200262

110423-218

11.8 ± 0.5*

Sarstedt

831810

6065081

13.4 ± 1.1*

TPP

90025

20150141

4.5 ± 1.3

  1. The results shown in Table represent 3 separate runs on each type of T-25 flasks. Therefore, the sem represent n = 3. For each run, 3–4 flasks of each brand are used
  2. * Indicates that fluid shear effect in flask is significantly greater (p < 0.05) than the non-shear value
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BMC Research Notes

ISSN: 1756-0500

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