Table 1 Primers: Virulence genes of Staphylococcus aureus
Serial no. | Primer pairs/ Target gene | Sequence | Length (bp) | Reaction conditions | Reference |
|---|---|---|---|---|---|
1 | icaA F | GACCTCGAAGTCAATAGAGGT | 814bp | Initial denaturation-94°C/2min \( \left. \begin{aligned} {\text{Denaturation}} - { 94}^\circ {\text{C}}/ 30{\text{ s}} \hfill \\ {\text{Annealing }} - { 46}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ {\text{Extension}} - { 72}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ \end{aligned} \right\}35\;{\text{cycles}} \) Final Extension- 72°C/ 7 min | [31] |
icaA R | CCCAGTATAACGTTGGATACC | ||||
2 | sea F | TTGGAAACGGTTAAAACGAA | 120bp | [27] | |
sea R | GAACCTTCCCATCAAAAACA | ||||
3 | seb F | TCGCATCAAACTGACAAACG | 478bp | [27] | |
seb R | GCAGGTACTCTATAAGTGCC | ||||
4 | eta F | CTAGTGCATTTGTTATTCAA | 119bp | Initial denaturation-94°C/2min \( \left. \begin{aligned} {\text{Denaturation}} - { 94}^\circ {\text{C}}/ 30{\text{ s}} \hfill \\ {\text{Annealing }} - { 46}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ {\text{Extension}} - { 72}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ \end{aligned} \right\}32\;{\text{cycles}} \) Final Extension- 72°C/ 7 min | [27] |
eta R | TGCATTGACACCATAGTACT | ||||
5 | etb F | ACGGCTATATACATTCAATT | 200bp | [27] | |
etb R | TCCATCGATAATATACCTAA | ||||
6 | tst F | ACCCCTGTTCCCTTATCATC | 326bp | [30] | |
tst R | TTTTCAGTATTTGTAACGCC | ||||
7 | cna F | GTCAAGCAGTTATTAACACCAGAC | 423bp | Initial denaturation-95°C/2min \( \left. \begin{aligned} {\text{Denaturation}} - { 95}^\circ {\text{C}}/ 30{\text{ s}} \hfill \\ {\text{Annealing }} - { 50}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ {\text{Extension}} - { 72}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ \end{aligned} \right\}32\;{\text{cycles}} \) Final Extension- 72°C/ 7 min | [32] |
cna R | AATCAGTAATTGCACTTTGTCCACTG | ||||
8 | fnbA F | GTGAAGTTTTAGAAGGTGGAAAGATTG | 643bp | [33] | |
fnbA R | GCTCTTGTAAGACCATTTTTCTTCAC | ||||
9 | femB F | TTACAGAGTTAACTGTTACC | 651bp | Initial denaturation-95°C/2min \( \left. \begin{aligned} {\text{Denaturation}} - { 95}^\circ {\text{C}}/ 30{\text{ s}} \hfill \\ {\text{Annealing }} - { 44}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ {\text{Extension}} - { 72}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ \end{aligned} \right\}35\;{\text{cycles}} \) Final Extension- 72°C/ 7 min | [29] |
femB R | ATACAAATCCAGCACGCTCT | ||||
10 | nuc F | GCGATTGATGGTGATACGGTT | 270bp | Initial denaturation-95°C/2min \( \left. \begin{aligned} {\text{Denaturation}} - { 95}^\circ {\text{C}}/ 30{\text{ s}} \hfill \\ {\text{Annealing }} - { 49}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ {\text{Extension}} - { 72}^\circ {\text{C}}/ 1 {\text{ min}} \hfill \\ \end{aligned} \right\}35\;{\text{cycles}} \) Final Extension- 72°C/ 7 min | |
nuc R | AGCCAAGCCTTGAACGAACTAAAGC |