Fig. 1

The sum of reads produced from both PCR replicates are depicted for wild-caught nymphs from Endymion and Thwartway Islands. With the exception of “no BLAST hit” sequences, the sum of reads depicted exceed the minimum identification confidence thresholds of 95% identity, a minimum overlap of query sequence to ≥ 100 nucleotides, and a depth of coverage of ≥ 50 reads for identification. All “no BLAST hit” sequences were unidentifiable by the “All BOLD Bin” reference library, likely attributed to non-target amplification. Amplified products from 51.57% (33/64) and 62.41% (98/157) of nymphs from Endymion and Thwartway respectively did not produce any reads that met the minimum confidence thresholds from either PCR replicate