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Table 1 Results of small angle X-ray scattering analysis to determine average (standard deviation) D-spacing and fibril diameter and results from the transmission electron microscopy image analysis to determine average (standard deviation) fibril diameter of sheep and cats’ corneas treated with 5% glutaraldehyde (G), 10% formalin (F), Triton X (T), and 0.9 % saline (S). P-values relate to a significance test between the various preservatives relative to the control untreated cornea sample values (C)

From: Pilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis

Species

Collagen properties

Preservative

Control

G

F

T

S

C

Sheep

D-spacing (nm)

64.96 (0.09)

65.16 (0.04)

65.58 (0.02)

65.36 (0.04)

65.42 (0.01)

P-value

< 0.05

< 0.05

< 0.05

0.06

–

SAXS fibril diameter (nm)

37.08 (0.23)

36.44 (0.24)

38.27 (0.32)

36.68 (0.20)

35.52 (0.15)

P-value

 < 0.05

 < 0.05

 < 0.05

< 0.05

–

Fibril diameter distribution (nm)

5.40 (0.45)

5.22 (0.20)

3.99 (0.34)

3.50 (0.36)

4.83 (0.31)

P-value

< 0.05

< 0.05

< 0.05

< 0.05

–

TEM fibril diameter

35.88 (3.27)

38.35 (3.28)

52.37 (10.51)

40.81 (5.69)

32.10 (2.70)

P-value

< 0.05

< 0.05

< 0.05

< 0.05

–

Cat

D-spacing (nm)

64.71 (0.06)

64.83 (0.06)

65.30 (0.11)

65.15 (0.01)

65.22 (0.01)

P-value

< 0.05

< 0.05

0.29

0.07

–

SAXS fibril diameter (nm)

40.50 (0.25)

39.18 (0.31)

41.73 (0.48)

38.50 (0.23)

37.26 (0.24)

P-value

< 0.05

< 0.05

< 0.05

< 0.05

–

Fibril diameter distribution (nm)

4.89 (0.28)

5.01 (0.21)

4.21 (0.37)

3.23 (0.37)

4.53 (0.12)

P-value

< 0.05

< 0.05

< 0.05

< 0.05

–

TEM fibril diameter

43.21 (5.12)

47.38 (5.74)

45.69 (7.65)

44.29 (6.43)

42.07 (4.13)

P-value

< 0.05

< 0.05

< 0.05

< 0.05

–