TY - JOUR AU - Ajayi, A. AU - Jolaiya, T. AU - Smith, S. I. PY - 2021 DA - 2021/03/09 TI - Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR—a pilot study JO - BMC Research Notes SP - 90 VL - 14 IS - 1 AB - Prompt diagnosis of Helicobacter pylori infection is essential for proper treatment and eradication of the pathogen because prolonged infection could lead to gastric cancer. Sensitive and cost effective diagnostic methods are key to guiding treatment options that will reduce mortality. This study was aimed at detecting H. pylori from biopsies of peptic ulcer patients. Real-time PCR using TaqMan and EvaGreen assays targeting 16S rRNA and ureA genes were used to detect H. pylori DNA extracted from 40 biopsy samples comprising 20 biopsies obtained from the antrum and 20 from the corpus of 20 patients undergoing endoscopy for duodenal ulcer investigation in Lagos, Nigeria. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-021-05505-y DO - 10.1186/s13104-021-05505-y ID - Ajayi2021 ER -